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Test ID RAIFA Antinuclear Antibodies, HEp-2 Substrate, IgG, with Reflex, Serum


Ordering Guidance


This algorithm is recommended for the initial evaluation of patients at risk for systemic lupus erythematosus, mixed connective tissue disease, and Sjogren syndrome.



Specimen Required


Supplies: Sarstedt Aliquot Tube, 5 mL (T914)

Collection Container/Tube:

Preferred: Serum gel

Acceptable: Red top

Submission Container/Tube: Plastic vial

Specimen Volume: 0.7 mL Serum

Collection Instructions: Centrifuge and aliquot serum into a plastic vial.


Secondary ID

622521

Useful For

Evaluation of patients suspected of having systemic autoimmune rheumatic disease (antinuclear antibody-associated rheumatic diseases or connective tissue diseases), especially systemic lupus erythematosus, mixed connective tissue disease and Sjogren syndrome

Reflex Tests

Test ID Reporting Name Available Separately Always Performed
RFLXP ANA Ab HEp-2 Reflex Panel No No

Testing Algorithm

If the human epithelial type 2 (HEp-2) antinuclear antibodies indirect immunofluorescence assay (IFA) result is positive with a titer of 1:80 or greater, then a titer and pattern will be reported.

 

If positive for a homogeneous, speckled, or dense fine speckled pattern, then confirmatory testing for double-stranded DNA antibodies (Ab), ribonucleoprotein Ab, Scl-70 Ab, Sm Ab, SS-A/Ro Ab, or SS-B/La Ab will be reflexed and performed at an additional charge. If confirmatory tests are negative, consideration of other associated antinuclear antibodies may be required for evaluation and confirmatory testing for these may be performed based on reported patterns or clinical suspicion at an additional charge.

Specimen Type

Serum

Specimen Stability Information

Specimen Type Temperature Time
Serum Refrigerated (preferred) 21 days
  Frozen  21 days

Reject Due To

Gross hemolysis Reject
Gross lipemia Reject
Gross icterus OK
Heat-treated specimen Reject

Reference Values

<1:80 (negative)

Day(s) Performed

Monday through Saturday

Report Available

3 to 4 days

Performing Laboratory

Mayo Clinic Laboratories in Rochester

CPT Code Information

86039