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HelpTest ID PENTS Pentobarbital, Serum
Useful For
Monitoring of pentobarbital therapy treatment
Performing Laboratory
Mayo Clinic Laboratories in Rochester
Specimen Type
Serum RedSpecimen Required
Supplies: Sarstedt Aliquot Tube, 5 mL (T914)
Collection Container/Tube: Red top (serum gel/SST are not acceptable)
Submission Container/Tube: Plastic vial
Specimen Volume: 1.5 mL
Collection Instructions:
1. Draw blood immediately before the next scheduled dose.
2. Within 2 hours of collection centrifuge and aliquot serum into a plastic vial.
Specimen Stability Information
| Specimen Type | Temperature | Time |
|---|---|---|
| Serum Red | Refrigerated (preferred) | 28 days |
| Ambient | 28 days | |
| Frozen | 28 days |
Reference Values
Therapeutic range
Hypnotic: 1.0-5.0 mcg/mL
Therapeutic coma: 20-50 mcg/mL
Reducing intracranial pressure: 30-40 mcg/mL
This degree of sedation requires artificial respiratory support.
Toxic concentration: >10 mcg/mL
Cutoff concentrations by gas chromatography mass spectrometry:
Pentobarbital: 0.5 ng/mL
Day(s) Performed
Thursday
CPT Code Information
80299
Report Available
3 to 9 daysReject Due To
| Gross hemolysis | OK |
| Gross lipemia | OK |
| Gross icterus | OK |
Forms
If not ordering electronically, complete, print, and send a Therapeutics Test Request (T831) with the specimen.