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Test ID NGPCM MayoComplete Plasma Cell Myeloma, Next-Generation Sequencing, Varies


Shipping Instructions


Bone marrow aspirate samples must arrive within 4 days of collection.



Specimen Required


Submit only 1 of the following specimens:

 

Preferred:

Specimen Type: Bone marrow aspirate

Container/Tube: Lavender or pink top (EDTA) or yellow top (ACD)

Specimen Volume: 2 mL

Collection Instructions:

1. Minimum plasma cell percentage is 5%.

2. Invert several times to mix bone marrow.

3. Send bone marrow specimen in original tube. Do not aliquot.

4. Label specimen as bone marrow.

5. Fresh specimen is required for this test, as testing is performed on sorted cells.

Specimen Stability Information: Ambient (preferred) 4 days/Refrigerate

Additional Information: To ensure minimum volume and concentration of DNA are met, the requested volume must be submitted. Testing may be canceled if DNA requirements are inadequate.

 

Specimen Type: Paraffin-embedded bone marrow clot

Container/Tube: Paraffin block

1. Send 1 hematoxylin and eosin-stained slide in addition to the paraffin block.

2. Minimum plasma cell percentage is 20%.

3. Required amount of tissue area is at least 25 mm(2).

4. Tissue should be fixed in 10% neutral-buffered formalin. Other fixatives are not acceptable.

5. Decalcified specimens (eg, bone marrow core biopsies) are not acceptable.

Specimen Stability Information: Ambient

Additional Information: If the quality of the specimen is poor or the plasma cell population is below 20%, testing should not be ordered. Testing may be canceled if DNA requirements are inadequate.

 

Acceptable:

Specimen Type: Tissue slide, bone marrow clot

Slides: 20 unstained slides

Container/Tube: Transport in plastic slide holders.

Collection Instructions:

1. Send 20 unstained, nonbaked slides with 5-micron thick sections of tissue and 1 hematoxylin and eosin-stained slide.

2. Minimum amount of plasma cell percentage is 20%.

4. Required amount of tissue area is at least 25 mm(2).

5. Tissue should be fixed in 10% neutral-buffered formalin. Other fixatives are not acceptable.

6. Decalcified specimens (eg, bone marrow core biopsies) are not acceptable.

Specimen Stability Information: Ambient

Additional Information: Testing may be canceled if resultant extracted DNA does not meet concentration requirements.


Forms

1. Hematopathology Patient Information (T676)

2. If not ordering electronically, complete, print, and send an Hematopathology/Cytogenetics Test Request (T726) with the specimen.

Secondary ID

618479

Useful For

Evaluating multiple myeloma at the time of diagnosis and at disease relapse or when changing clinical management to provide prognostic information and determine potential therapeutic implications

Reflex Tests

Test ID Reporting Name Available Separately Always Performed
CSPMM NGPCM Pre-Analysis Cell Sorting, BM No No

Testing Algorithm

For a list of the genes and exons targeted by this assay, see Targeted Genes Interrogated by MayoComplete Plasma Cell Myeloma Next-Generation Sequencing.

Specimen Type

Varies

Specimen Stability Information

Specimen Type Temperature Time
Varies Varies

Reject Due To

Gross hemolysis Reject
Gross lipemia OK
Specimens that have been decalcified (all methods) Reject
Bone marrow core biopsies Reject
Paraffin shavings Reject
Frozen tissue Reject
Extracted DNA Reject
Moderately to severely clotted bone marrow aspirate Reject

Reference Values

An interpretive report will be provided.

Day(s) Performed

Monday through Friday

Report Available

16 to 21 days

Performing Laboratory

Mayo Clinic Laboratories in Rochester

CPT Code Information

81450